Miles Morales / Spider-Man (voice)
Peter B. Parker / Spider-Man (voice)
Gwen Stacy / Spider-Woman (voice)
Uncle Aaron / Prowler (voice)
Jefferson Davis (voice)
Aunt May (voice)
Rio Morales (voice)
Mary Jane (voice)
Peter Porker / Spider-Ham (voice)
Peni Parker (voice)
Peter Parker / Spider-Man Noir (voice)
Olivia Liv Octavius / Doc Ock (voice)
Wilson Fisk / Kingpin (voice)
Peter Parker / Spider-Man (voice)
Miss Calleros (voice)
Brooklyn Visions Security Guard (voice)
Interesting Person #1 (voice)
Interesting Person #2 (voice)
Stan (voice)
Green Goblin / Last Dude (voice)
Scorpion (voice)
Tombstone (voice)
Scientist in Cafeteria (voice)
Vanessa Fisk (voice)
Brooklyn Friend (voice)
Brooklyn Visions Student (voice)
Brooklyn Visions Teacher (voice)
Brooklyn Bystander (voice)
Additional Voices (voice)
Jonathan Ohnn / Spot (voice)